Medical applications of electron paramagnetic resonance (EPR)
Low frequency EPR (< 1 GHz) (EPR – electron paramagnetic resonance) has developed rapidly in recent years and this opens for a new field of research with fascinating medical applications. Examples are non-invasive true quantitative in vivo imaging of tissue oxygenation in cancer tumours and in vivo imaging of reactive oxygen species in atherosclerotic plaques. Initial attempts of in vivo EPR have been performed in human volunteers.
The in vivo EPR and EPR imaging (EPRI) laboratory at Linköping University has recently showed the possibility of e.g. EPR imaging of oxidative stress in human ex vivo atherosclerotic plaques and the possibility of intrarenal EPR oximetry. In addition; new methods have been developed for high precision quantifications of reactive oxygen species (ROS) in biopsies, cell cultures and blood.
The forthcoming aim of this project is to continue the development of in vivo EPR and EPR oximetry towards clinical use. The specific aims are: (1) To develop novel methods for in vivo quantitative imaging of oxygen pressure. (2) In vivo imaging of reactive oxygen species. (3) To study the dependence of oxygen pressure and reactive oxygen species on prognosis and treatment outcome for e.g. cancer, atherosclerosis and diabetes in collaboration with research groups specialised in each of these subjects respectively.
Figure 1. Photograph of a section obtained from a human carotid atherosclerotic plaque immediately after cryosection, incubation with a spin trap and after covered with Parafilm (panel A). Panel B shows the EPRI image obtained after reconstruction. Note that the lumen does not give any EPRI intensity as expected and a few areas with high intensity are present. Panel C shows the areas with highest EPRI intensity fused onto the photograph. Panel D shows the histology of an adjacent slice with the high signalling areas superimposed as dashed outlines. L; lumen, C; lipid core. Scale bar correspond to 2 mm. (Gustafsson, H., Hallbeck, M., Lindgren, M., Kolbun, N., Jonson, M., Engström, M., de Muinck, E. and Zachrisson, H.)
Pronounced kidney hypoxia precedes albuminuria in type 1 diabetic mice
S. Franzén, L. Pihl, H. Gustafsson, F. Palm
In press 2016: Journal of the American Society of Nephrology
Visualization of oxidative stress in ex vivo biopsies using electron paramagnetic resonance imaging
Gustafsson, H., Hallbeck, M., Lindgren, M., Kolbun, N., Jonson, M., Engström, M., de Muinck, E. and Zachrisson, H.
Magnetic Resonance in Medicine 2014: doi: 10.1002/mrm.25267
A high precision method for quantitative measurement of reactive oxygen species in biopsies
K. Berg, M. Ericsson, M. Lindgren, H. Gustafsson
PLoS ONE 9(3): e90964. doi:10.1371/journal.pone.0090964
Repetitive measurements of intrarenal oxygenation in vivo using L-band Electron Paramagnetic Resonance
S. Franzén, L. Pihl, N. Khan, F. Palm, H. Gustafsson
Advances in Experimental Medicine and Biology 812 (2014) 135-141
Senast uppdaterad: 2016-04-29